| 1. | This morphology was slightly different from that of mouse eg cells
这与经典的小鼠胚胎生殖细胞的形态有所不同。 |
| 2. | The stem cells derived from pgcs are called embryonic germ cells ( eg cells )
来源于pgcs的干细胞称胚胎生殖细胞。 |
| 3. | This experiment shows this eg cell line is pluripotent , germline transmission remains to be confirmed
结果表明该eg细胞系具有多能性，是否可以进入种系有待进一步验证。 |
| 4. | Pgc and eg cells derived from pgc characterized by a typical colony appearance with very scarce individual cell
对传2代、 4代的eg细胞进行akp染色，所得的eg细胞为akp阳性细胞。 |
| 5. | To date , eg cells with proven germ - line transmission have been completely established only in the mouse with embryonic stem cells ( es cells )
迄今为止仅在小鼠上获得pgcs来源的真正意义上的胚胎干细胞( escells ) ，即具有参与生殖系传递能力的es细胞。 |
| 6. | In this study , the putative eg cell culture media was supplemented with lif , bfgf , scf and igf - 1 . as a result , the ideal dose of lif , bfgf , scf and igf - 1 was : 100iu / ml , 10ng / ml , 10ng / ml , 10ng / ml
而随着培养时间的延长，添加lif 、 bfgf 、 scf 、及igf - 1组表现的优势越来越明显，对促进类eg集落的持续增多效果最佳。 |
| 7. | Eg cells of the 2th and 4th passage were akp ( alkaline phosphate ) positive . when cultured on degenerated feeder layers or in suspension , eg c ells formed embryoid bodies ( ebs ) in vitro
当eg细胞脱离饲养层悬浮培养，或在衰老的饲养层上延迟培养时，发现eg细胞或单个存在，或聚集成团，形成类似于早期胚胎的囊状胚体结构 |
| 8. | With the increase of passage , the total of colonies decreased , but the number of classical nest - like or island - like eg cell colonies relatively increased . the growth of eg cells was dependent on the feeder layer
传代培养的eg细胞对饲养层有依赖性， icr品系小鼠pgc细胞和由其传代后获得的eg细胞呈明显的群聚性，以单个存在状态的较少 |
| 9. | In the experiment , in case of the proliferative capability of eg cells , the composition of 0 . 25 % trypsin + 0 . 04edta was optimal . the growth behavior of icr murine eg cells from pgc was observed
传代过程中，以0 . 25胰酶与0 . 04 edta联合作用效果较好，对eg细胞的综合损伤最小，离散传代后eg细胞活力比较强， eg克隆出现最多，最高传至6代。 |
| 10. | A , pgcs collected from fetuses were immediately injected into the embryos after isolation ; b , pgcs were cultured on gelatin - coated multidishes for up to 24 h untill injection ; c , eg cells of 0 - 6 passages
将新鲜和培养24h的pgc 、 6代前的eg细胞进行显微注射。从42头供体猪获得564枚胚胎。 419枚胚胎注射pgc eg细胞，与未注射的145枚胚胎搭配混合后分别移植给22头受体猪。 |
